Isolation of Rutin from Neocarya macrophylla leaves (Sabine) Prance (Chrysobalanaceae)

Abstract

Neocarya macrophylla is a medicinal plant which belong to the Chrysobalanaceae family. It has many ethnomedicinal uses such as treatment of snake bite, pain and inflammation. In this work, a flavonoid diglycoside was isolated from the ethylacetate fraction of the methanol leaf extract of the plant. The compound was isolated using a combination of silica gel and sephadex LH-20 column chromatography. Using 1H and 13C-NMR spectroscopy followed by  comparison with previously established data in the literature, the structure of the compound elucidated was, 3′, 4′, 5, 7-tetrahydroxyflavone-3-(α-L-rhamnopyronosyl-(1→6)-β-D-glucopyranose) (quercetin-3-O-rutinoside) known as  Rutin. It is a flavonoid diglycoside that is used to treat and manage a variety of conditions such as pain and inflammation. To the best of our knowledge, this is the first report of isolation and characterization of rutin from the leaves of N. macrophylla.