Preliminary Study of the Phytochemical Constituents and Antimicrobial Activity of the Ethanolic Extract of Papaya (Carica Papaya) Seed

Abstract

This study undertook a comprehensive examination of the phytochemical constituents and antimicrobial 
activities of Carica papaya seed. The seeds were subjected to ethanol extraction, and the resulting extract 
was meticulously screened for the presence of phytochemicals. Subsequent testing assessed the extract 
efficacy against two bacterial strains: Escherichia coli and Staphylococcus aureus. The results of the study 
revealed that the extract of Carica papaya seeds is replete with an array of phytochemicals, including 
alkaloids, flavonoids, tannins, saponins, and terpenoids. Notably, the extract exhibited pronounced 
antimicrobial activity against both bacterial strains. The minimum inhibitory concentration (MIC) of the 
extract was determined to be 6.25 mg/mL against S. aureus and 12.50 mg/mL against E. coli. The findings 
of this study conclusively demonstrate that Carica papaya seed possess significant antimicrobial properties, 
rendering them a promising natural remedy for the treatment of diseases caused by bacteria. Furthermore, 
this study underscores the importance of exploring the potential medicinal applications of Carica papaya 
seed and their constituents.