The biological activities of secondary metabolites of Parinari macrophylla Sabine

Abstract

 The secondary metabolites of Parinari macrophylla Sabine of the family Chrysobalanaceae was
 examined. The fruits and stem were successively extracted with hexane, ethyl acetate and methanol.
 Phytochemical analyses of plant extracts revealed the presence of phenolic compounds, alkaloid and
 glycosides. Plant extracts were evaluated for biological activity using the brine shrimp lethality bioassay
 method. The results showed the presence of cyctotoxic compounds and the following LC50 values (at 95%
 confidence interval) of 201, 241 and 500 were obtained for hexane, ethyl acetate and methanol extracts
 respectively. Chromatographic purification of the ethyl acetate extract gave six fractions of which
 fractions A, B and F LC50 20, 125 and 300 respectively. Antimicrobial test showed plants extracts to be
 active against Staphylocccus aureus, Escherichia coli, Salmonella typhi, candidas albicans and
 Pseudomonas aeruginosa with m.i.c value 20 g/ml. IR studies of plant extracts showed presence of
 hydroxyl and carbonyl functional groups